isotype control ab  (InvivoGen)

Journal: Journal of Genetic Engineering & Biotechnology

Article Title: Co-expression of HIF1A with multi-drug transporters (P-GP, MRP1, and BCRP) in chemoresistant breast, colorectal, and ovarian cancer cells

doi: 10.1016/j.jgeb.2025.100496

Figure Lengend Snippet: ABC transporter and HIF1A co-expression profiles in chemoresistant cancerous and paired control breast tissues. (A) ABC transporters and HIF1A co-expression in chemoresistant breast cancer tissue. (B) ABC transporter and HIF1A co-expression in adjacent control tissues. In (A) and (B) the expressions of P-GP, MRP1, and BCRP are shown in green and HIF1A in red, respectively. The scale bar is 25 µm. (C), (D) and (E) show the correlations of P-GP, MRP1 and BCRP expressions with HIF1A, respectively, in breast tissues. The X-axis in (C), (D), and (E) denotes the relative ratio of HIF1A expression to total cells, whereas the Y-axis denotes the relative ratio of P-GP, MRP1, and BCRP expression in total cells, respectively. The slopes indicate linear relationship between transporters and HIF1A expression. (F) Comparison of ABC transporters and HIF1A co-expression in chemoresistant breast cancer and adjacent control tissues. The X-axis represents P-GP and HIF1A (green), MRP1 and HIF1A (orange), as well as BCRP and HIF1A (blue) co-expressions. The Y-axis denotes the relative percentage of ABC transporter and HIF1A co-expression. The Wilcoxon signed-rank test for paired samples was conducted to validate the significance of differences for all types of co-expression. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: To ensure the specific binding ability of 10 Abs for ABC transporters, non-specific rabbit (DA1E) monoclonal Ab IgG XP® isotype control (3900; Cell Signaling Technology®; diluted 1:1500) was applied on an individual section (Data not shown).This incubation lasted overnight at 4 °C.

Techniques: Expressing, Control, Comparison

Journal: Journal of Genetic Engineering & Biotechnology

Article Title: Co-expression of HIF1A with multi-drug transporters (P-GP, MRP1, and BCRP) in chemoresistant breast, colorectal, and ovarian cancer cells

doi: 10.1016/j.jgeb.2025.100496

Figure Lengend Snippet: ABC transporter and HIF1A co-expression profiles in chemoresistant cancerous and paired control colorectal tissues. (A) ABC transporters and HIF1A co-expression in chemoresistant colorectal cancer tissues. (B) ABC transporters and HIF1A co-expression in adjacent control tissues. In (A) and (B) the expressions of P-GP, MRP1, and BCRP are shown in green and HIF1A in red, respectively. The scale bar is 25 µm. (C), (D) and (E) show the correlations of P-GP, MRP1 and BCRP expressions with HIF1A, respectively, in colorectal tissues. The X-axis in (C), (D), and (E) denotes the relative ratio of HIF1A expression to total cells. The Y-axis in (C), (D), and (E) denotes the relative ratio of P-GP, MRP1, and BCRP expression to total cells, respectively. The slopes indicate the linear relationship between transporters and HIF1A expression. (F) Comparison of ABC transporter and HIF1A co-expression in chemoresistant colorectal cancer and adjacent control tissues. The X-axis represents P-GP and HIF1A (green), MRP1 and HIF1A (orange), and BCRP and HIF1A (blue) co-expressions. The Y-axis denotes the relative percentage of ABC transporter and HIF1A co-expression. The Wilcoxon signed-rank test for paired samples was conducted to validate the significance of differences for all types of co-expression. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: To ensure the specific binding ability of 10 Abs for ABC transporters, non-specific rabbit (DA1E) monoclonal Ab IgG XP® isotype control (3900; Cell Signaling Technology®; diluted 1:1500) was applied on an individual section (Data not shown).This incubation lasted overnight at 4 °C.

Techniques: Expressing, Control, Comparison

Journal: Journal of Genetic Engineering & Biotechnology

Article Title: Co-expression of HIF1A with multi-drug transporters (P-GP, MRP1, and BCRP) in chemoresistant breast, colorectal, and ovarian cancer cells

doi: 10.1016/j.jgeb.2025.100496

Figure Lengend Snippet: ABC transporter and HIF1A co-expression profiles in chemoresistant cancerous and paired control ovarian tissues. (A) ABC transporters and HIF1A co-expression in chemoresistant ovarian cancer tissues. (B) ABC transporters and HIF1A co-expression in adjacent control tissues. In (A) and (B) the expressions of P-GP, MRP1, and BCRP are shown in green and HIF1A in red, respectively. The scale bar is 25 µm. (C), (D) and (E) show the correlations of P-GP, MRP1 and BCRP expressions with HIF1A, respectively, in colorectal tissues in ovarian tissues. The X-axis in (C), (D), and (E) denotes the relative ratio of HIF1A expression to total cells. The Y-axis in (C), (D), and (E) denotes the relative ratio of P-GP, MRP1, and BCRP expression to total cells, respectively. The slopes indicate the linear relationship between transporters and HIF1A expression. (F) Comparison of ABC transporter and HIF1A co-expression in chemoresistant ovarian cancer and adjacent control tissues. The X-axis represents P-GP and HIF1A (green), MRP1 and HIF1A (orange), and BCRP and HIF1A (blue) co-expressions. The Y-axis denotes the relative percentage of ABC transporter and HIF1A co-expression. The Wilcoxon signed-rank test for paired samples was conducted to validate the significance of differences for all types of co-expression. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: To ensure the specific binding ability of 10 Abs for ABC transporters, non-specific rabbit (DA1E) monoclonal Ab IgG XP® isotype control (3900; Cell Signaling Technology®; diluted 1:1500) was applied on an individual section (Data not shown).This incubation lasted overnight at 4 °C.

Techniques: Expressing, Control, Comparison